Myc-gamma3/gamma2SIL | Addgene 121433 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

Myc-gamma3/gamma2SIL

catalog :

121433

product information

Catalog Number :

121433

Product Name :

Myc-gamma3/gamma2SIL

article :

doi
id	28197201
pubmed_id

bacterial resistance :

Ampicillin

cloning :

backbone

pCDNA3.1

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth strain :

GABAA receptor expression (chimeric rat gamma 3 subunit whose large intracellular loop has been replaced with the large intracellular loop of the rat gamma 2 short subunit) Myc-tag near N-terminus

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	XhoI
cloning_site_5	NheI
promoter
sequencing_primer_3
sequencing_primer_5	unknown
site_3_destroyed
site_5_destroyed

entrez_gene

aliases
gene	Gabrg2
id	29709

aliases
gene	Gabrg3
id	79211

genbank_ids

mutation

name

GABRG3/GABRG2 SIL

shRNA_sequence

size

species

10116

Rattus norvegicus

tags

location	N terminal on insert
tag	cMyc epitope EQKLISEEDL inserted between fourth and fifth amino acids of mature protein after signal peptide is cleaved

plasmid copy :

Rat gamma 3 and rat gamma 2 genes were originally cloned in Peter Seeburg s lab in Heidelberg. Subcloning into different plasmids, tagging and making the chimeric constructs was done in Angel de Blas's lab.

resistance markers :

4182

tags :

High Copy