35S:5mARF17 pLBR19 | Addgene 12073 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

35S:5mARF17 pLBR19

catalog :

12073

citations: 1

Reference
Mallory A, Bartel D, Bartel B. MicroRNA-directed regulation of Arabidopsis AUXIN RESPONSE FACTOR17 is essential for proper development and modulates expression of early auxin response genes. Plant Cell. 2005;17:1360-75 pubmed

product information

Catalog Number :

12073

Product Name :

35S:5mARF17 pLBR19

article :

doi	10.1105/tpc.105.031716
id	719
pubmed_id	15829600

bacterial resistance :

Ampicillin

cloning :

backbone

pLBR19

backbone_mutation

backbone_origin

(pLBR19 is derived from pUC19, but contains the CaMV 35S promote

backbone_size

5500

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Plant Expression

growth notes :

To generate 35S:5mARF17, a 3.3 kb EcoR1-BamH1 fragment of the 6.6 kb 5mARF17 pBluescript clone was subcloned into pLBR19 downstream from the duplicated enhancer of CaMV 35S RNA promoter. See sequence for full insert sequence.

origin :

pi :

alt_names

auxin response transcription factor 17

ARF17

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	BamHI
promoter
sequencing_primer_3	CaMV term rev (5'-TTG ATA GAA GTA TTT TAC AAA TAC-3')
sequencing_primer_5	35Spromoter (5'-CTA TCC TTC GCA AGA CCC TTC-3')
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	AT1G77850, F28K19.6, F28K19_6, auxin response factor 17
gene	ARF17
id	844120

genbank_ids

mutation

miR160-resistant version of ARF17 has five silent mutations within the miR160-complementary domain of an ARF17 genomic clone, thereby increasing the number of mismatches between miR160 and the ARF17 mRNA from one in wild type to six.

name

5mARF17

shRNA_sequence

size

3300

species

3702

Arabidopsis thaliana