This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLEX-FKBP-Kras-Blasticidin
catalog :
120714
citations: 1
Reference
Kovalski J, Bhaduri A, Zehnder A, Neela P, Che Y, Wozniak G, et al. The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2. Mol Cell. 2019;73:830-844.e12 pubmed publisher
product information
Catalog Number :
120714
Product Name :
pLEX-FKBP-Kras-Blasticidin
article :
doi10.1016/j.molcel.2018.12.001
id28197097
pubmed_id30639242
bacterial resistance :
Ampicillin
cloning :
backbonepLEX
backbone_mutationPuromycin resistance cassette swapped for blasticidin resistance.
backbone_originThermo Scientific
backbone_size11000
promoterCMV
sequencing_primer_3ATATAGACAAACGCACACCGGCCT
sequencing_primer_5CACCAAAATCAACGGGACTT
vector_types
Mammalian Expression
Lentiviral
growth strain :
Expresses membrane-targeted recruiter protein in mammalian cells & for virus production
origin :
37
pi :
alt_names
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3ATATAGACAAACGCACACCGGCCT
sequencing_primer_5CACCAAAATCAACGGGACTT
site_3_destroyed
site_5_destroyed
entrez_gene
aliases'C-K-RAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, K-Ras, K-Ras 2, KI-RAS, KRAS1, KRAS2, NS, NS3, OES, RALD, RASK2, c-Ki-ras, c-Ki-ras2
geneKRAS
id3845
genbank_ids
mutationamino acids 158-188
nameKRAS4B
shRNA_sequence
size1202
species
9606
Homo sapiens
tags
locationN terminal on insert
tagFKBP
locationN terminal on insert
tagmCherry
plasmid copy :
FKBP-mCherry-KrasC30 insert was cloned out of Addgene plasmid #72900.
resistance markers :
249
tags :
Unknown
terms :
Blasticidin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA