This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
5mARF17 pBluescript
catalog :
12069
citations: 1
Reference
Mallory A, Bartel D, Bartel B. MicroRNA-directed regulation of Arabidopsis AUXIN RESPONSE FACTOR17 is essential for proper development and modulates expression of early auxin response genes. Plant Cell. 2005;17:1360-75 pubmed
product information
Catalog Number :
12069
Product Name :
5mARF17 pBluescript
article :
doi10.1105/tpc.105.031716
id719
pubmed_id15829600
bacterial resistance :
Ampicillin
cloning :
backbonepBluescript II SK+
backbone_mutation
backbone_originStratagene
backbone_size3000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
See sequence for full insert sequence. See article for details of site-directed mutagenesis.
origin :
37
pi :
alt_names
auxin response transcription factor 17
ARF17
cloning
clone_methodRestriction Enzyme
cloning_site_3SpeI
cloning_site_5EcoRI
promoter
sequencing_primer_3
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAT1G77850, F28K19.6, F28K19_6, auxin response factor 17
geneARF17
id844120
genbank_ids
mutationmiR160-resistant version of ARF17 has five silent mutations within the miR160-complementary domain of an ARF17 genomic clone, thereby increasing the number of mismatches between miR160 and the ARF17 mRNA from one in wild type to six.
name5mARF17
shRNA_sequence
size6600
species
3702
Arabidopsis thaliana
tags
resistance markers :
21
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA