This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
5mCUC1 pBluescript
catalog :
12065
citations: 1
Reference
Mallory A, Dugas D, Bartel D, Bartel B. MicroRNA regulation of NAC-domain targets is required for proper formation and separation of adjacent embryonic, vegetative, and floral organs. Curr Biol. 2004;14:1035-46 pubmed
product information
Catalog Number :
12065
Product Name :
5mCUC1 pBluescript
article :
doi10.1016/j.cub.2004.06.022
id720
pubmed_id15202996
bacterial resistance :
Ampicillin
cloning :
backbonepBluescript II SK+
backbone_mutation
backbone_originStratagene
backbone_size3000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
CAAATAACTCTGCTGCTCATACCG-3' and 5mCUC1 reverse 5'-CGGTATGAGCAGCAGAGTTATTTGAAAAACATGAAACATGCTCCGTCGCAAAGGTATTGATGATCGG-3'. See sequence for the full insert sequence. See article for cloning details.
5'-CCGATCATCAATACCTTTGCGACGGAGCATGTTTCA
TGTTTTT
The CUC1 plasmid (addgene # 12064) was used as a starting material. The sequence of the primer pair used for CUC1 mutagenesis is 5mCUC1 forward
origin :
37
pi :
alt_names
CUC1
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5BamHI
promoter
sequencing_primer_3
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAT3G15170, ANAC054, ATNAC1, Arabidopsis NAC domain containing protein 54, CUP-SHAPED COTYLEDON1
geneCUC1
id820748
genbank_ids
mutationmiR-resistant CUC1. Made by introducing five silent mutations within the miR164-complementarity domain of a CUC1 genomic clone.
nameCUC1 mutant
shRNA_sequence
size3700
species
3702
Arabidopsis thaliana
tags
resistance markers :
21
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA