This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
EF1a_HOXB6_P2A_Hygro_Barcode
catalog :
120454
citations: 1
Reference
Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, et al. Mapping Cellular Reprogramming via Pooled Overexpression Screens with Paired Fitness and Single-Cell RNA-Sequencing Readout. Cell Syst. 2018;7:548-555.e8 pubmed publisher
product information
Catalog Number :
120454
Product Name :
EF1a_HOXB6_P2A_Hygro_Barcode
article :
doi10.1016/j.cels.2018.10.008
id28197093
pubmed_id30448000
bacterial resistance :
Ampicillin
cloning :
backboneUnknown
backbone_mutation
backbone_origin
backbone_size9514
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Lentiviral
growth notes :
5' cloning site: BamHI (not destroyed), 3' cloning site: BamHI (not destroyed).
growth strain :
Barcoded lentiviral vector to express HOXB6 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
inserts :
Stbl3
origin :
37
pi :
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterEF1a
sequencing_primer_3
sequencing_primer_55'-ttctcaagcctcagacagtgg-3'
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHOX2, HOX2B, HU-2, Hox-2.2
geneHOXB6
id3216
genbank_ids
mutation
nameHOXB6
shRNA_sequence
size672
species
9606
Homo sapiens
tags
resistance markers :
4273
tags :
High Copy
terms :
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA