This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Fibronectin-human-mutated V2 O-Glycosylation site
catalog :
120405
citations: 1
Reference
Sens C, Altrock E, Rau K, Klemis V, von Au A, Pettera S, et al. An O-Glycosylation of Fibronectin Mediates Hepatic Osteodystrophy Through α4β1 Integrin. J Bone Miner Res. 2017;32:70-81 pubmed publisher
product information
Catalog Number :
120405
Product Name :
Fibronectin-human-mutated V2 O-Glycosylation site
article :
doi10.1002/jbmr.2916
id28197084
pubmed_id27427791
bacterial resistance :
Kanamycin
cloning :
backbonepMAX-Cloning
backbone_mutation
backbone_origin
backbone_size2868
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Clone pFN 2. O-Gly_sp_1. It was used to study the role of the various fibronectin isoforms in transfected eucaryotic cells and to produce the isoforms.
growth strain :
enables eukaryotic expression of human plasma fibronectin in which the second O-glycosylation site was mutated from threonine to serine
origin :
37
pi :
alt_names
Oncofetal fibronectin-V2 mutation
Construct without mutation is referred to as oncofetal fibronectin
cloning
clone_methodRestriction Enzyme
cloning_site_3Unknown
cloning_site_5Unknown
promoterCMV
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCIG, ED-B, FINC, FN, FNZ, GFND, GFND2, LETS, MSF, SMDCF
geneFN1
id2335
genbank_ids
mutationContains complete variable region with a mutation from threonine to serine at position 1974/1975 at V2 O-Glycosylation site to prevent O-Glycosylation
nameFibronectin
shRNA_sequence
size6914
species
9606
Homo sapiens
tags
resistance markers :
4268
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA