Fibronectin-human-EDA in pMAX | Addgene 120402 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

Fibronectin-human-EDA in pMAX

catalog :

120402

citations: 2

Reference
Melamed S, Zaffryar Eilot S, Nadjar Boger E, Aviram R, Zhao H, Yaseen Badarne W, et al. Initiation of fibronectin fibrillogenesis is an enzyme-dependent process. Cell Rep. 2023;42:112473 pubmed publisher
Rossnagl S, Altrock E, Sens C, Kraft S, Rau K, Milsom M, et al. EDA-Fibronectin Originating from Osteoblasts Inhibits the Immune Response against Cancer. PLoS Biol. 2016;14:e1002562 pubmed publisher

product information

Catalog Number :

120402

Product Name :

Fibronectin-human-EDA in pMAX

article :

doi	PBIOLOGY-D-16-00754 [pii]
id	28197083
pubmed_id	27653627

bacterial resistance :

Kanamycin

cloning :

backbone

pMAX-Cloning

backbone_mutation

backbone_origin

backbone_size

2868

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Clone EDA sp44. It was used to study the role of the various fibronectin isoforms in transfected eucaryotic cells and to produce the isoforms. See also Fibronectins containing extradomain A or B enhance osteoblast differentiation via distinct integrins. J Biol Chem. 2017 May 12;292(19):7745-7760. https://www.ncbi.nlm.nih.gov/pubmed/28325836

growth strain :

enables eukaryotic expression of human EDA (or EIIIA) containing fibronectin based on plasma fibronectin sequence

origin :

pi :

alt_names

Human fibronectin containing complete variable region and EDA (or EIIIA) domain

EIIIA fibronectin, cellular fibronectin

cloning

clone_method	Restriction Enzyme
cloning_site_3	Unknown
cloning_site_5	Unknown
promoter	CMV
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CIG, ED-B, FINC, FN, FNZ, GFND, GFND2, LETS, MSF, SMDCF
gene	FN1
id	2335

genbank_ids

mutation

name

EDA-fibronectin

shRNA_sequence

size

7184

species

9606

Homo sapiens