This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pAAV-U6-Diras2_1-hSyn::mCherry.3xFLAG-WPRE
catalog :
120393
citations: 1
Reference
Grünewald L, Chiocchetti A, Weber H, Scholz C, Schartner C, Freudenberg F, et al. Knockdown of the ADHD Candidate Gene Diras2 in Murine Hippocampal Primary Cells. J Atten Disord. 2019;:1087054718822129 pubmed publisher
product information
Catalog Number :
120393
Product Name :
pAAV-U6-Diras2_1-hSyn::mCherry.3xFLAG-WPRE
article :
doi10.1177/1087054718822129
id28197078
pubmed_id30623719
bacterial resistance :
Ampicillin
cloning :
backbonepAAV
backbone_mutation
backbone_origin
backbone_size4000
promoterU6
sequencing_primer_3ATGCGCAATTTGGGGAATGG
sequencing_primer_5GCCAACTCCATCACTAGGGG
vector_types
AAV
RNAi
growth notes :
The original backbone was published in Candemir et al. Eur Neuropsychopharmacol. 2016 (PMID: 26861996)
growth strain :
pAAV plasmid expressing Diras2 shRNA1 under the U6 promoter and mCherry.3XFLAG under the hSyn promoter
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BspQI
cloning_site_5BspQI
promoterU6
sequencing_primer_3ATGCGCAATTTGGGGAATGG
sequencing_primer_5GCCAACTCCATCACTAGGGG
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliases2900052J15Rik, AI414999
geneDiras2
id68203
genbank_ids
NM_001024474
mutation
nameDiras2 shRNA
shRNA_sequence
size53
species
10090
Mus musculus
tags
locationC terminal on backbone
tagmCherry.3XFLAG
locationC terminal on backbone
tagmCherry.3XFLAG
resistance markers :
4269
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA