This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET-32a(+)-TGFB1
catalog :
120361
citations: 1
Reference
Kuo H, Gao X, DeKeyser J, Fetterman K, Pinheiro E, Weddle C, et al. Negligible-Cost and Weekend-Free Chemically Defined Human iPSC Culture. Stem Cell Reports. 2020;14:256-270 pubmed publisher
product information
Catalog Number :
120361
Product Name :
pET-32a(+)-TGFB1
article :
doi10.1016/j.stemcr.2019.12.007
id28197051
pubmed_id31928950
bacterial resistance :
Ampicillin
cloning :
backbonepET-32a (+)
backbone_mutation
backbone_originNovagen
backbone_size5900
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Please visit https://www.biorxiv.org/content/10.1101/685503v2 for BioRxiv preprint
growth strain :
Produce human recombinant TGFB1 in DE3 cells
inserts :
BL21 Star/DE3
origin :
37
pi :
DPD1, IBDIMDE, LAP, TGF-beta1, TGFB, TGFbetageneTGFB1id7040genbank_idsmutationCodon optimized for E. Coli productionnameTransforming Growth Factor- 1shRNA_sequencesize339species
9606
Homo sapiens
100
Synthetic
tags
locationN terminal on insert
tag6x His
locationN terminal on insert
tagS-Tag

Promotersequencing_pr
imer_3ctagcataaccccttggggcctct
aaacgggtcttgaggggttttttg<
td>sequencing_primer_5taatacga
ctcactataggsite_3_des
troyedsite_5
_destroyedtd>entrez_geneble>
aliasesCED,
alt_names
TGFB1
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5BamHI
promoterT7
plasmid copy :
Printed sequence service from SGI
resistance markers :
4285
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA