This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pAAV-RSV-GFP-U6-Rosa26 gRNA (SpyCas9 scaffold)
catalog :
120296
citations: 1
Reference
Hoffmann M, Aschenbrenner S, Grosse S, Rapti K, Domenger C, Fakhiri J, et al. Cell-specific CRISPR-Cas9 activation by microRNA-dependent expression of anti-CRISPR proteins. Nucleic Acids Res. 2019;: pubmed publisher
product information
Catalog Number :
120296
Product Name :
pAAV-RSV-GFP-U6-Rosa26 gRNA (SpyCas9 scaffold)
article :
doi10.1093/nar/gkz271
id28197054
pubmed_id30982889
bacterial resistance :
Ampicillin
cloning :
backbonescAAV
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
AAV
CRISPR
growth notes :
Please visit https://www.biorxiv.org/content/10.1101/480384v1 for BioRxiv preprint
growth strain :
AAV vector; encodes GFP as well as a U6-driven Rosa26-targeting gRNA (SpyCas9 scaffold)
origin :
37
pi :
alt_names
GFP
cloning
clone_methodRestriction Enzyme
cloning_site_3 Unknown
cloning_site_5Unknown
promoter
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameRosa26 gRNA (SpCas9 scaffold)
shRNA_sequenceThe spacer sequence of the Rosa-26 guideRNA is 5 -GACTCCAGTCTTTCTAGAAGA-3
size
species
32644
Other
tags
resistance markers :
4114
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA