This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET-28a(+)-FGF2-K128N
catalog :
120284
citations: 1
Reference
Kuo H, Gao X, DeKeyser J, Fetterman K, Pinheiro E, Weddle C, et al. Negligible-Cost and Weekend-Free Chemically Defined Human iPSC Culture. Stem Cell Reports. 2020;14:256-270 pubmed publisher
product information
Catalog Number :
120284
Product Name :
pET-28a(+)-FGF2-K128N
article :
doi10.1016/j.stemcr.2019.12.007
id28197051
pubmed_id31928950
bacterial resistance :
Kanamycin
cloning :
backbonepET-28a (+)
backbone_mutation
backbone_originNovagen
backbone_size5900
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Please visit https://www.biorxiv.org/content/10.1101/685503v2 for BioRxiv preprint
growth strain :
Produce human recombinant FGF2 K128N in DE3 cells
inserts :
BL21 Star/DE3
origin :
37
pi :
FGF-2, FGFB, HBGF-2geneFGF2id2247genbank_idsmutationCodon optimized for E. Coli productionnameBasic fibroblast growth factor 2shRNA_sequencesize476species
9606
Homo sapiens
100
Synthetic
tags
locationN terminal on insert
tag6x His

promotersequencing_pr
imer_3ctagcataaccccttggggcctct
aaacgggtcttgaggggttttttg<
td>sequencing_primer_5taatacga
ctcactataggsite_3_des
troyedsite_5
_destroyedtd>entrez_geneble>
aliasesBFGF,
alt_names
FGF2-K128N
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5BamHI
promoterT7
plasmid copy :
Printed sequence service from SGI
resistance markers :
4285
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA