This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pAG70 BDNF 3'UTR
catalog :
12021
citations: 1
Reference
Farh K, Grimson A, Jan C, Lewis B, Johnston W, Lim L, et al. The widespread impact of mammalian MicroRNAs on mRNA repression and evolution. Science. 2005;310:1817-21 pubmed
product information
Catalog Number :
12021
Product Name :
pAG70 BDNF 3'UTR
article :
doi10.1126/science.1121158
id717
pubmed_id16308420
bacterial resistance :
Ampicillin
cloning :
backbonepIS2
backbone_mutation
backbone_originpIS2 derived from pRL-SV40 (Promega)
backbone_size3700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Luciferase
growth notes :
BDNF 3'UTR in renilla luciferase reporter plasmid. Wild-type microRNA recognition sites (seed matches): 37-42 and 207-212 and 1139-1144.
origin :
37
pi :
alt_names
BDNF
brain-derived neurotrophic factor
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5SacI
promoter
sequencing_primer_3
sequencing_primer_5EBV rev primer (GTGGTTTGTCCAAACTCATC)
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesANON2, BULN2
geneBDNF
id627
genbank_ids
NM_170735
mutation
nameBDNF 3'UTR
shRNA_sequence
size1141
species
9606
Homo sapiens
tags
resistance markers :
21
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA