This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
TOM20*-SBP-GFP
catalog :
120173
citations: 2
Reference
Zhang Z, Denans N, Liu Y, Zhulyn O, Rosenblatt H, Wernig M, et al. Optogenetic manipulation of cellular communication using engineered myosin motors. Nat Cell Biol. 2021;23:198-208 pubmed publisher
Guardia C, De Pace R, Sen A, Saric A, Jarnik M, Kolin D, et al. Reversible association with motor proteins (RAMP): A streptavidin-based method to manipulate organelle positioning. PLoS Biol. 2019;17:e3000279 pubmed publisher
product information
Catalog Number :
120173
Product Name :
TOM20*-SBP-GFP
article :
doi10.1371/journal.pbio.3000279
id28197027
pubmed_id31100061
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-N1
backbone_mutationContains mutation A206K for producing monomeric eGFP
backbone_originClontech
backbone_size4715
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expresses a chimera of the mitochondria-targeting signal of TOM20, SBP tag and GFP (fluorescent tag)
origin :
37
pi :
alt_names
TOM20
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3EGFPN1 rev
sequencing_primer_5CMV fwd
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesMAS20, MOM19, TOM20
geneTOMM20
id9804
genbank_ids
mutationTruncated TOM20: 1-30 aa
nameMitochondrial import receptor subunit TOM20 homolog
shRNA_sequence
size201
species
9606
Homo sapiens
tags
locationC terminal on insert
tagSBP
locationC terminal on backbone
tageGFP
resistance markers :
1784
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA