This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSF4 TetCMV 5'TOP intron 20xGCN4 Renilla FKBP Stop 24xMS2v5 SV40 CTE polyA
catalog :
119946
citations: 3
Reference
Sidhaye J, Trepte P, Sepke N, Novatchkova M, Schutzbier M, D xfc rnberger G, et al. Integrated transcriptome and proteome analysis reveals posttranscriptional regulation of ribosomal genes in human brain organoids. elife. 2023;12: pubmed publisher
Matějů D, Eichenberger B, Voigt F, Eglinger J, Roth G, Chao J. Single-Molecule Imaging Reveals Translation of mRNAs Localized to Stress Granules. Cell. 2020;183:1801-1812.e13 pubmed publisher
Wilbertz J, Voigt F, Horvathova I, Roth G, Zhan Y, Chao J. Single-Molecule Imaging of mRNA Localization and Regulation during the Integrated Stress Response. Mol Cell. 2019;73:946-958.e7 pubmed publisher
product information
Catalog Number :
119946
Product Name :
pSF4 TetCMV 5'TOP intron 20xGCN4 Renilla FKBP Stop 24xMS2v5 SV40 CTE polyA
article :
doi10.1016/j.molcel.2018.12.006
id28197008
pubmed_id30661979
bacterial resistance :
Ampicillin
cloning :
backbonepSF4
backbone_mutation
backbone_origin
backbone_size10500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Bacterial Expression
growth notes :
Please visit https://www.biorxiv.org/content/10.1101/332502v1 for BioRxiv preprint Note plasmid contains 24xGCN4 repeats and not 20xGCN4 as described in the name.
growth strain :
Expresses 5'TOP-SunTag-Renilla mRNA reporter in mammalian cells; note plasmid contains 24xGCN4 repeats
origin :
37
pi :
alt_names
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterTetCMV
sequencing_primer_3
sequencing_primer_5AATACGACTCACTATAGGC
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameRenilla luciferase
shRNA_sequence
size930
species
32644
Other
Renilla reniformis
tags
location
tag5'TOP sequence
locationN terminal on insert
tag24xGCN4 repeats (SunTag)
locationC terminal on insert
tagFKBP
location
tag24xMS2v5 stem loops in 3'UTR
resistance markers :
2309
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA