pSF4 TetCMV intron 20xGCN4 Renilla FKBP Stop 24xMS2v5 SV40 CTE polyA

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product summary

company name :

Addgene

product type :

cDNA

product name :

pSF4 TetCMV intron 20xGCN4 Renilla FKBP Stop 24xMS2v5 SV40 CTE polyA

catalog :

119945

citations: 3

Reference
Dave P, Roth G, Griesbach E, Matějů D, Hochstoeger T, Chao J. Single-molecule imaging reveals translation-dependent destabilization of mRNAs. Mol Cell. 2023;: pubmed publisher
Matějů D, Eichenberger B, Voigt F, Eglinger J, Roth G, Chao J. Single-Molecule Imaging Reveals Translation of mRNAs Localized to Stress Granules. Cell. 2020;183:1801-1812.e13 pubmed publisher
Wilbertz J, Voigt F, Horvathova I, Roth G, Zhan Y, Chao J. Single-Molecule Imaging of mRNA Localization and Regulation during the Integrated Stress Response. Mol Cell. 2019;73:946-958.e7 pubmed publisher

product information

Catalog Number :

119945

Product Name :

pSF4 TetCMV intron 20xGCN4 Renilla FKBP Stop 24xMS2v5 SV40 CTE polyA

article :

doi	10.1016/j.molcel.2018.12.006
id	28197008
pubmed_id	30661979

bacterial resistance :

Ampicillin

cloning :

backbone

pSF4

backbone_mutation

backbone_origin

backbone_size

10500

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Bacterial Expression

growth notes :

Please visit https://www.biorxiv.org/content/10.1101/332502v1 for BioRxiv preprint Note plasmid contains 24xGCN4 repeats and not 20xGCN4 as described in the name.

growth strain :

Expresses SunTag-Renilla mRNA reporter in mammalian cells; note plasmid contains 24xGCN4 repeats

origin :

pi :

alt_names

cloning

clone_method	Unknown
cloning_site_3
cloning_site_5
promoter	TetCMV
sequencing_primer_3
sequencing_primer_5	AATACGACTCACTATAGGC
site_3_destroyed
site_5_destroyed

entrez_gene

genbank_ids

mutation

name

Renilla luciferase

shRNA_sequence

size

930

species

32644

Other

Renilla reniformis