This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
sup35-C653R
catalog :
1198
citations: 1
Reference
True H, Berlin I, Lindquist S. Epigenetic regulation of translation reveals hidden genetic variation to produce complex traits. Nature. 2004;431:184-7 pubmed
product information
Catalog Number :
1198
Product Name :
sup35-C653R
article :
doi10.1038/nature02885
id50
pubmed_id15311209
bacterial resistance :
Ampicillin
cloning :
backbonepRS306
backbone_mutation
backbone_originHieter
backbone_size4381
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth notes :
yeast integration plasmid for replacement of Sup35 with mutant. Cut with AgeI for integration. Additional Reference: Bradley ME, Bagriantsev S, Vishveshwara N and Liebman SW. Guanidine reduces stop codon read-through caused by missense mutations in SUP35 or SUP45. Yeast 20, 625-632 (2003).
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5EcoRI
promoter
sequencing_primer_3
sequencing_primer_5n/a
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYDR172W, GST1, PNM2, SAL3, SUF12, SUP2, SUP36
geneSUP35
id851752
genbank_ids
NC_001136
mutationchanged cys 653 to arg
nameSup35p
shRNA_sequence
size2057
species
4932
Saccharomyces cerevisiae
tags
resistance markers :
293
tags :
High Copy
terms :
URA3
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA