This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
mOrange-imp
catalog :
119720
citations: 1
| Reference |
|---|
Nakagawa C, Nishimura S, Senda Murata K, Sugimoto K. A rapid and simple method of evaluating the dimeric tendency of fluorescent proteins in living cells using a truncated protein of importin α as fusion tag. Biosci Biotechnol Biochem. 2012;76:388-90 pubmed
|
product information
Catalog Number :
119720
Product Name :
mOrange-imp
article :
| doi | 10.1271/bbb.110677 |
| id | 28196947 |
| pubmed_id | 22313767 |
bacterial resistance :
Kanamycin
cloning :
| backbone | pmOrange-C1 | |
| backbone_mutation | NheI-BglII DNA fragment encoding EGFP of pEGFP is replaced by the DNA fragment encoding mOrange, which is amplified by PCR using pRSET B mOrange (provided by Dr. Roger Y. Tsien). | |
| backbone_origin | not commercially available one. Note that Eco47III, AgeI, BspE1 are missing | |
| backbone_size | ||
| promoter | ||
| sequencing_primer_3 | ||
| sequencing_primer_5 | ||
| vector_types |
|
growth notes :
pmOrange-imp was constructed by replacing the AgeI-BglII fragment encoding DsRed of pDsRed-imp (Addgene plasmid 119679) with mOrange derived from pRSET-B-mOrange (provided by Professor Roger Y. Tsien, UCSD).
growth strain :
Expresses mOrange-tagged human importin in mammalian cells
origin :
37
pi :
|
resistance markers :
| 4176 |
tags :
High Copy
terms :
| Neomycin (select with G418) |
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments