This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pEGFP-imp
catalog :
119718
citations: 1
Reference
Nakagawa C, Nishimura S, Senda Murata K, Sugimoto K. A rapid and simple method of evaluating the dimeric tendency of fluorescent proteins in living cells using a truncated protein of importin α as fusion tag. Biosci Biotechnol Biochem. 2012;76:388-90 pubmed
product information
Catalog Number :
119718
Product Name :
pEGFP-imp
article :
doi10.1271/bbb.110677
id28196947
pubmed_id22313767
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP- C2
backbone_mutationpEGFP-delat C2 is constructed by ligation of blunt-ended HindIII site of pEGFP-C1, resulting in the C2-type reading frame
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
1-kb fragment of the EcoRI-XhoI fragment of DsRed-imp alpha (Addgene plasmid 119679) was cloned into the EcoRI-SalI sites of pEGFP- C2, a frame-shifted derivative of pEGFP-C1.
growth strain :
Expresses EGFP-tagged human importin in mammalian cells
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5EcoRI
promoterCMV
sequencing_primer_3
sequencing_primer_5n/a
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesIPOA1, PTAC58, QIP2, RCH1, SRP1-alpha, SRP1alpha
geneKPNA2
id3838
genbank_ids
mutationcontains amino acids 251-529
nameimportin
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagEGFP
resistance markers :
4176
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA