This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pGRE NPD-GFP
catalog :
1197
citations: 1
Reference
Patino M, Liu J, Glover J, Lindquist S. Support for the prion hypothesis for inheritance of a phenotypic trait in yeast. Science. 1996;273:622-6 pubmed
product information
Catalog Number :
1197
Product Name :
pGRE NPD-GFP
article :
doi10.1126/science.273.5275.622
id80
pubmed_id8662547
bacterial resistance :
Ampicillin
cloning :
backbonepUC18 w/ GRE promoter
backbone_mutation
backbone_origin
backbone_size2800
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth notes :
2 micron yeast plasmid Please note that BamHI and SpeI are NOT single cutters in this plasmid. See the Addgene digest above.
origin :
37
pi :
alt_names
Sup35
cloning
clone_methodRestriction Enzyme
cloning_site_3SpeI
cloning_site_5BamHI
promoter
sequencing_primer_3M13 Rev
sequencing_primer_5M13/pUC Forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYDR172W, GST1, PNM2, SAL3, SUF12, SUP2, SUP36
geneSUP35
id851752
genbank_ids
NC_001136
mutationfused N-terminal Sup35 (~1-253aa) to GFP
nameN-terminus of Sup35 fused to GFP
shRNA_sequence
size1200
species
4932
Saccharomyces cerevisiae
tags
location
tagGFP
resistance markers :
293
tags :
High Copy
terms :
URA3
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA