This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBS537 tet-hCMV-GFPcre
catalog :
11960
citations: 1
Reference
Le Y, Miller J, Sauer B. GFPcre fusion vectors with enhanced expression. Anal Biochem. 1999;270:334-6 pubmed
product information
Catalog Number :
11960
Product Name :
pBS537 tet-hCMV-GFPcre
article :
doi10.1006/abio.1999.4110
id691
pubmed_id10334853
bacterial resistance :
Ampicillin
cloning :
backbonena
backbone_mutation
backbone_origin
backbone_size4300
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Cre/Lox
growth notes :
pBS537 carries a GFPcre fusion gene under the control of a synthetic promoter that can be regulated by a synthetic tet repressor/activator protein. In the presence of doxycycline expression is dramatically turned on to high levels. The GFP moiety carries the S65T mutation for enhanced fluorescence, but is not codon-optimized for mammalian expression.
origin :
37
pi :
alt_names
cre
cloning
clone_methodRestriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5na
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesP1_gp003
genecre
id2777477
genbank_ids
mutation
nameGFP-cre
shRNA_sequence
size1850
species
bacteriophage P1
tags
resistance markers :
440
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA