This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBS595 tet-hCMV-EGFPcre
catalog :
11957
citations: 1
| Reference |
|---|
Le Y, Miller J, Sauer B. GFPcre fusion vectors with enhanced expression. Anal Biochem. 1999;270:334-6 pubmed
|
product information
Catalog Number :
11957
Product Name :
pBS595 tet-hCMV-EGFPcre
article :
| doi | 10.1006/abio.1999.4110 |
| id | 691 |
| pubmed_id | 10334853 |
bacterial resistance :
Ampicillin
cloning :
| backbone | na | ||
| backbone_mutation | |||
| backbone_origin | |||
| backbone_size | 5200 | ||
| promoter | |||
| sequencing_primer_3 | |||
| sequencing_primer_5 | |||
| vector_types |
|
growth notes :
pBS595 differs from pBS597 in having the GCSF A(n) instead of the MT-I A(n). pBS597 carries the EGFPcre fusion gene under the control of a synthetic promoter that can be regulated by a synthetic tet repressor/activator protein. In the presence of doxycycline expression is dramatically turned on to high levels. The GFP moiety of the fusion gene carries the S65T mutation for enhanced fluorescence and is codon-optimised for higher level expression. Two related constructs are the promoterless vector pBS592 and the vector pBS598 having constitutive high level expression.
origin :
37
pi :
|
resistance markers :
| 440 |
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments