This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBS594 promoterless EGFPcre
catalog :
11956
citations: 1
Reference
Le Y, Miller J, Sauer B. GFPcre fusion vectors with enhanced expression. Anal Biochem. 1999;270:334-6 pubmed
product information
Catalog Number :
11956
Product Name :
pBS594 promoterless EGFPcre
article :
doi10.1006/abio.1999.4110
id691
pubmed_id10334853
bacterial resistance :
Ampicillin
cloning :
backbonena
backbone_mutation
backbone_origin
backbone_size3900
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Cre/Lox
growth notes :
pBS594 differs from pBS592 in having the MT-I A(n) instead of the GCSF A(n). MT-I A(n) is the mouse metallothionein MT-I region including the polyadenylation site and several introns. pBS592 carries the EGFPcre fusion gene in a promoterless vector with several available restriction sites. The GFP moiety of the fusion gene carries the S65T mutation for enhanced fluorescence and is codon-optimised for higher level expression. Two related constructs are the pBS597 with a tet-controlled promoter and the vector pBS598 having constitutive high level expression.
origin :
37
pi :
alt_names
cre
cloning
clone_methodRestriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5na
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesP1_gp003
genecre
id2777477
genbank_ids
mutation
nameEGFP-cre
shRNA_sequence
size1800
species
bacteriophage P1
tags
resistance markers :
440
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA