This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBS505 EF1alpha-EGFPcre*
catalog :
11955
citations: 1
| Reference |
|---|
Le Y, Miller J, Sauer B. GFPcre fusion vectors with enhanced expression. Anal Biochem. 1999;270:334-6 pubmed
|
product information
Catalog Number :
11955
Product Name :
pBS505 EF1alpha-EGFPcre*
article :
| doi | 10.1006/abio.1999.4110 |
| id | 691 |
| pubmed_id | 10334853 |
bacterial resistance :
Ampicillin
cloning :
| backbone | na | ||
| backbone_mutation | |||
| backbone_origin | |||
| backbone_size | 6600 | ||
| promoter | |||
| sequencing_primer_3 | |||
| sequencing_primer_5 | |||
| vector_types |
|
growth notes :
pBS505 is similar to pBS598 except the NcoI site within EGFPcre is replaced by a cluster of 6 bp cutters (BspE1, BglII, XhoI, SacI, HindIII, EcoRI, PstI, SalI, NotI). pBS598 carries the EGFPcre fusion gene under the control of the strong elongation factor EF1alpha promoter. This promoter expresses at high levels in most cell types, including embryonic stem (ES) cells. However its use in transgenic mice is not recommended: the promoter is often silenced in a transgene setting. The GFP moiety of the fusion gene carries the S65T mutation for enhanced fluorescence and is codon-optimised for higher level expression. Note: pBS513 (EF1alpha-cre) is very similar to pBS598 but carries the wt cre gene and thus serves as the non-fluorescent counterpart of pBS598.
origin :
37
pi :
|
resistance markers :
| 440 |
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments