pBS598 EF1alpha-EGFPcre | Addgene 11923 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pBS598 EF1alpha-EGFPcre

catalog :

11923

citations: 6

Reference
Wong Dilworth L, Rodilla Ramirez C, Fox E, Restel S, Stockhammer A, Adarska P, et al. STED imaging of endogenously tagged ARF GTPases reveals their distinct nanoscale localizations. J Cell Biol. 2023;222: pubmed publisher
Mahadevan J, Jha A, Rudolph J, Bowerman S, Narducci D, Hansen A, et al. Dynamics of endogenous PARP1 and PARP2 during DNA damage revealed by live-cell single-molecule imaging. iScience. 2023;26:105779 pubmed publisher
Schultz M, Schache K, Azaria R, Kuiper E, Erwood S, Ivakine E, et al. Species-specific differences in NPC1 protein trafficking govern therapeutic response in Niemann-Pick type C disease. JCI Insight. 2022;7: pubmed publisher
Hamline M, Corcoran C, Wamstad J, Miletich I, Feng J, Lohr J, et al. OFCD syndrome and extraembryonic defects are revealed by conditional mutation of the polycomb-group repressive complex 1.1 (PRC1.1) gene BCOR. Dev Biol. 2020;: pubmed publisher
Youmans D, Schmidt J, Cech T. Live-cell imaging reveals the dynamics of PRC2 and recruitment to chromatin by SUZ12-associated subunits. Genes Dev. 2018;32:794-805 pubmed publisher
Le Y, Miller J, Sauer B. GFPcre fusion vectors with enhanced expression. Anal Biochem. 1999;270:334-6 pubmed

product information

Catalog Number :

11923

Product Name :

pBS598 EF1alpha-EGFPcre

article :

doi	10.1006/abio.1999.4110
id	691
pubmed_id	10334853

bacterial resistance :

Ampicillin

cloning :

backbone

backbone_mutation

backbone_origin

backbone_size

6600

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Cre/Lox

growth notes :

pBS598 carries the EGFPcre fusion gene under the control of the strong elongation factor EF1alpha promoter. This promoter expresses at high levels in most cell types, including embryonic stem (ES) cells. However its use in transgenic mice is not recommended: the promoter is often silenced in a transgene setting. The GFP moiety of the fusion gene carries the S65T mutation for enhanced fluorescence and is codon-optimised for higher level expression. Note: pBS513 (EF1alpha-cre) is very similar to pBS598 but carries the wt cre gene and thus serves as the non-fluorescent counterpart of pBS598.

origin :

pi :

alt_names

cre

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5	na
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	P1_gp003
gene	cre
id	2777477

genbank_ids

mutation

name

EGFP-cre

shRNA_sequence

size

1800

species

bacteriophage P1