This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBS500 EF1alpha-GFPcre
catalog :
11920
citations: 3
Reference
Baek S, Kerjan G, Bielas S, Lee J, Fenstermaker A, Novarino G, et al. Off-target effect of doublecortin family shRNA on neuronal migration associated with endogenous microRNA dysregulation. Neuron. 2014;82:1255-1262 pubmed publisher
Moon H, Youn Y, Pemble H, Yingling J, Wittmann T, Wynshaw Boris A. LIS1 controls mitosis and mitotic spindle organization via the LIS1-NDEL1-dynein complex. Hum Mol Genet. 2014;23:449-66 pubmed publisher
Gagneten S, Le Y, Miller J, Sauer B. Brief expression of a GFP cre fusion gene in embryonic stem cells allows rapid retrieval of site-specific genomic deletions. Nucleic Acids Res. 1997;25:3326-31 pubmed
product information
Catalog Number :
11920
Product Name :
pBS500 EF1alpha-GFPcre
article :
doi10.1093/nar/25.16.3326
id692
pubmed_id9241248
bacterial resistance :
Ampicillin
cloning :
backbonena
backbone_mutation
backbone_origin
backbone_size5000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Cre/Lox
growth notes :
pBS500 carries a GFPcre fusion gene under the control of the elongation factor 1-alpha promoter. The GFP moiety carries the S65T mutation for enhanced fluorescence, but is not codon-optimized for mammalian expression. pBS500 expresses well in murine embryonic stem (ES) cells but this is not the case for the plasmid from which it is derived, pBS448.
origin :
37
pi :
alt_names
cre
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5BamHI
promoter
sequencing_primer_3
sequencing_primer_5na
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesP1_gp003
genecre
id2777477
genbank_ids
mutation
nameGFP-cre
shRNA_sequence
size1850
species
bacteriophage P1
tags
resistance markers :
440
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA