This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBS448 RSV-GFPcre
catalog :
11919
citations: 1
| Reference |
|---|
Gagneten S, Le Y, Miller J, Sauer B. Brief expression of a GFP cre fusion gene in embryonic stem cells allows rapid retrieval of site-specific genomic deletions. Nucleic Acids Res. 1997;25:3326-31 pubmed
|
product information
Catalog Number :
11919
Product Name :
pBS448 RSV-GFPcre
article :
| doi | 10.1093/nar/25.16.3326 |
| id | 692 |
| pubmed_id | 9241248 |
bacterial resistance :
Ampicillin
cloning :
| backbone | na | ||
| backbone_mutation | |||
| backbone_origin | |||
| backbone_size | 4400 | ||
| promoter | |||
| sequencing_primer_3 | |||
| sequencing_primer_5 | |||
| vector_types |
|
growth notes :
pBS448 carries a GFP-cre fusion gene under the control of the Rous sarcoma virus (RSV) LTR/promoter. The GFP moiety carries the S65T mutation for enhanced fluorescence, but is not codon-optimized for mammalian expression. Higher level expression can be obtained using the EF1-alpha promoter plasmid pBS500 having the same structural fusion gene. Use of the GFPcre fusion gene demonstrated that the Cre protein carries endogenous determinants that target the protein efficiently to the nucleus of eukaryotic cells. Thus the addition of an extraneous NLS is not required. The GFP-tagged cre gene is a convenient tool for directly observing the tissue-specificity of cre expression in transgenic mice.
origin :
37
pi :
|
resistance markers :
| 440 |
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
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