pBS513 EF1alpha-cre | Addgene 11918 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pBS513 EF1alpha-cre

catalog :

11918

citations: 5

Reference
Battulin N, Korablev A, Ryzhkova A, Smirnov A, Kabirova E, Khabarova A, et al. The human EF1a promoter does not provide expression of the transgene in mice. Transgenic Res. 2022;31:525-535 pubmed publisher
Li J, Li Y, Pawlik K, Napierala J, Napierala M. A CRISPR-Cas9, Cre-lox, and Flp-FRT Cascade Strategy for the Precise and Efficient Integration of Exogenous DNA into Cellular Genomes. CRISPR J. 2020;3:470-486 pubmed publisher
Mikulka C, Dearborn J, Benítez B, Strickland A, Liu L, Milbrandt J, et al. Cell-autonomous expression of the acid hydrolase galactocerebrosidase. Proc Natl Acad Sci U S A. 2020;117:9032-9041 pubmed publisher
Zenchak J, Palmateer B, Dorka N, Brown T, Wagner L, Medendorp W, et al. Bioluminescence-driven optogenetic activation of transplanted neural precursor cells improves motor deficits in a Parkinson's disease mouse model. J Neurosci Res. 2018;: pubmed publisher
Le Y, Miller J, Sauer B. GFPcre fusion vectors with enhanced expression. Anal Biochem. 1999;270:334-6 pubmed

product information

Catalog Number :

11918

Product Name :

pBS513 EF1alpha-cre

article :

doi	10.1006/abio.1999.4110
id	691
pubmed_id	10334853

bacterial resistance :

Ampicillin

cloning :

backbone

backbone_mutation

backbone_origin

backbone_size

6600

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Cre/Lox

growth notes :

pBS513 carries the wt cre gene under the control of the elongation factor-1 alpha (EF-1 alpha) promoter. The construct carries an upstream Kozak modification to increase expression. The resulting construct provides very strong expression in many different types of cells, including murine embryonic stem (ES) cells. For cell culture work this construct is recommended as a replacement for the CMV-cre construct pBS185 as it provides much stronger expression. However, pBS513 is not suitable for use in transgenic mice as such EF-1 alpha transgene constructs are often inactive in many tissues. Please note: The following enzymes are NOT single cutters: PstI, AflIII, and SacI. We do not suggest using these enzymes for cloning.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5	na
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	P1_gp003
gene	cre
id	2777477

genbank_ids

mutation

name

cre

shRNA_sequence

size

1100

species

bacteriophage P1