This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBS185 CMV-Cre
catalog :
11916
citations: 17
Reference
Coppiello G, Barlab xe9 P, Moya J xf3 dar M, Abizanda G, Pogontke C, Barreda C, et al. Generation of heart and vascular system in rodents by blastocyst complementation. Dev Cell. 2023;58:2881-2895.e7 pubmed publisher
Fini M, Monks J, Li M, Gerasimovskaya E, Paucek P, Wang K, et al. Macrophage Xanthine Oxidoreductase Links LPS Induced Lung Inflammatory Injury to NLRP3 Inflammasome Expression and Mitochondrial Respiration. bioRxiv. 2023;: pubmed publisher
Uno N, Takata S, Komoto S, Miyamoto H, Nakayama Y, Osaki M, et al. Panel of human cell lines with human/mouse artificial chromosomes. Sci Rep. 2022;12:3009 pubmed publisher
Hong C, Cohen B. Genomic environments scale the activities of diverse core promoters. Genome Res. 2021;: pubmed publisher
Joshi L, Knudsen D, Piñeyro P, Dhakal S, Renukaradhya G, Diel D. Protective Efficacy of an Orf Virus-Vector Encoding the Hemagglutinin and the Nucleoprotein of Influenza A Virus in Swine. Front Immunol. 2021;12:747574 pubmed publisher
Abizanda G, López Muneta L, Linares J, Ramos L, Baraibar Churio A, Bobadilla M, et al. Local Preirradiation of Infarcted Cardiac Tissue Substantially Enhances Cell Engraftment. Int J Mol Sci. 2021;22: pubmed publisher
Villette V, Chavarha M, Dimov I, Bradley J, Pradhan L, Mathieu B, et al. Ultrafast Two-Photon Imaging of a High-Gain Voltage Indicator in Awake Behaving Mice. Cell. 2019;179:1590-1608.e23 pubmed publisher
Dong M, Liu X, Evert K, Utpatel K, Peters M, Zhang S, et al. Efficacy of MEK inhibition in a K-Ras-driven cholangiocarcinoma preclinical model. Cell Death Dis. 2018;9:31 pubmed publisher
Agerholm M, Dall M, Jensen B, Prats C, Madsen S, Basse A, et al. Perturbations of NAD+ salvage systems impact mitochondrial function and energy homeostasis in mouse myoblasts and intact skeletal muscle. Am J Physiol Endocrinol Metab. 2018;314:E377-E395 pubmed publisher
Ade C, Derbes R, Wagstaff B, Linker S, White T, Deharo D, et al. Evaluating different DNA binding domains to modulate L1 ORF2p-driven site-specific retrotransposition events in human cells. Gene. 2018;642:188-198 pubmed publisher
He Q, Wang H, Cheng T, Yuan W, Ma Y, Jiang Y, et al. Genetic Correction and Hepatic Differentiation of Hemophilia B-specific Human Induced Pluripotent Stem Cells. Chin Med Sci J. 2017;32:135-144 pubmed publisher
Yim N, Ryu S, Choi K, Lee K, Lee S, Choi H, et al. Exosome engineering for efficient intracellular delivery of soluble proteins using optically reversible protein-protein interaction module. Nat Commun. 2016;7:12277 pubmed publisher
Somarelli J, Schaeffer D, Marengo M, Bepler T, Rouse D, Ware K, et al. Distinct routes to metastasis: plasticity-dependent and plasticity-independent pathways. Oncogene. 2016;35:4302-11 pubmed publisher
Lyu S, Kwon H. Preparation of cell-permeable Cre recombinase by expressed protein ligation. BMC Biotechnol. 2015;15:7 pubmed publisher
Horstick E, Jordan D, Bergeron S, Tabor K, Serpe M, Feldman B, et al. Increased functional protein expression using nucleotide sequence features enriched in highly expressed genes in zebrafish. Nucleic Acids Res. 2015;43:e48 pubmed publisher
Ju H, Calvisi D, Moon H, Baek S, Ribback S, Dombrowski F, et al. Transgenic mouse model expressing P53(R172H), luciferase, EGFP, and KRAS(G12D) in a single open reading frame for live imaging of tumor. Sci Rep. 2015;5:8053 pubmed publisher
Sauer B, Henderson N. Targeted insertion of exogenous DNA into the eukaryotic genome by the Cre recombinase. New Biol. 1990;2:441-9 pubmed
product information
Catalog Number :
11916
Product Name :
pBS185 CMV-Cre
article :
doi
id678
pubmed_id2288914
bacterial resistance :
Ampicillin
cloning :
backbonena
backbone_mutation
backbone_origin
backbone_size6000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Cre/Lox
growth notes :
pBS185 carries the wt cre gene under the control of the major immediate early promoter and enhancer of human cytomegalovirus (hCMV). This is a strong promoter that gives fairly generalized expression. However, it is not a strong promoter in embryonic stem (ES) cells. The construct also carries the mouse metallothionein MT-I region including the polyadenylation site and several introns. For higher level expression in general and for better expression in ES cells plasmid pBS513 is superior. The sequence shown includes cre and the metallothionein region.
growth strain :
Mammalian expression of Cre recombinase driven by the human cytomegalovirus IE promoter
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5na
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesP1_gp003
genecre
id2777477
genbank_ids
mutation
namecre
shRNA_sequence
size1100
species
bacteriophage P1
tags
resistance markers :
440
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA