pPAGFP-VSVG | Addgene 11915 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pPAGFP-VSVG

catalog :

11915

citations: 3

Reference
Fricke F, Beaudouin J, Eils R, Heilemann M. One, two or three? Probing the stoichiometry of membrane proteins by single-molecule localization microscopy. Sci Rep. 2015;5:14072 pubmed publisher
Fricke F, Malkusch S, Wangorsch G, Greiner J, Kaltschmidt B, Kaltschmidt C, et al. Quantitative single-molecule localization microscopy combined with rule-based modeling reveals ligand-induced TNF-R1 reorganization toward higher-order oligomers. Histochem Cell Biol. 2014;142:91-101 pubmed publisher
Presley J, Cole N, Schroer T, Hirschberg K, Zaal K, Lippincott Schwartz J. ER-to-Golgi transport visualized in living cells. Nature. 1997;389:81-5 pubmed

product information

Catalog Number :

11915

Product Name :

pPAGFP-VSVG

article :

doi	10.1038/38001
id	689
pubmed_id	9288971

bacterial resistance :

Kanamycin

cloning :

backbone

pEGFP-N1

backbone_mutation

backbone_origin

Clontech

backbone_size

4733

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

The EGFP (Clontech Laboratories, Inc., Palo, Alto, CA) contains a valine immediately after the start codon that is not found in the wild type sequence. However, to avoid confusion with previously published work on GFP mutants, the wild type residue numbers are used. The Kozak sequence at the beginning of the GFP encoding region of the pEGFP-N1 plasmid from Clontech has been disrupted. Reference for the A206K mutation is Zacharias, D. A., Violin, J. D., Newton, A. C., and Tsien, R. Y. (2002) Science 296, 913-6. Reference for PAGFP is Patterson, G. H., and Lippincott-Schwartz, J. (2002) Science 297, 1873-7.

origin :

pi :

alt_names

Vesicular stomatitus virus G protein

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	BglII
promoter
sequencing_primer_3	EGFP-N
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

genbank_ids

mutation

(L64F/T65S/V163A/T203H). The PAGFP also contains an A206K mutation to disrupt dimerization. The EGFP (Clontech Laboratories, Inc., Palo, Alto, CA) contains a valine immediately after the start codon that is not found in the wild type sequence. However, to avoid confusion with previously published work on GFP mutants, the wild type residue numbers are used.

name

VSVG

shRNA_sequence

size

1500

species