pCerulean-VSVG | Addgene 11913 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCerulean-VSVG

catalog :

11913

citations: 4

Reference
Etienne L, Joshi P, Dingle L, Huang E, Grzesik P, Desai P. Visualization of herpes simplex virus type 1 virions using fluorescent colors. J Virol Methods. 2017;241:46-51 pubmed publisher
Spear J, Koborssy D, Schwartz A, Johnson A, Audhya A, Fadool D, et al. Kv1.3 contains an alternative C-terminal ER exit motif and is recruited into COPII vesicles by Sec24a. BMC Biochem. 2015;16:16 pubmed publisher
Buffone A, Mondal N, Gupta R, McHugh K, Lau J, Neelamegham S. Silencing Î±1,3-fucosyltransferases in human leukocytes reveals a role for FUT9 enzyme during E-selectin-mediated cell adhesion. J Biol Chem. 2013;288:1620-33 pubmed publisher
Presley J, Cole N, Schroer T, Hirschberg K, Zaal K, Lippincott Schwartz J. ER-to-Golgi transport visualized in living cells. Nature. 1997;389:81-5 pubmed

product information

Catalog Number :

11913

Product Name :

pCerulean-VSVG

article :

doi	10.1038/38001
id	689
pubmed_id	9288971

bacterial resistance :

Kanamycin

cloning :

backbone

pECFP-N1

backbone_mutation

backbone_origin

Clontech

backbone_size

4733

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Cerulean ws developed by Piston lab. Reference is Rizzo, M. A., Springer, G. H., Granada, B., and Piston, D. W. (2004) Nat Biotechnol 22, 445-9. The EGFP (Clontech Laboratories, Inc., Palo, Alto, CA) contains a valine immediately after the start codon that is not found in the wild type sequence. However, to avoid confusion with previously published work on GFP mutants, the wild type residue numbers are used. The Kozak sequence at the beginning of the GFP encoding region of the pEGFP-N1 plasmid from Clontech has been disrupted.

origin :

pi :

alt_names

Vesicular stomatitus virus G protein

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	BglII
promoter
sequencing_primer_3
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

genbank_ids

mutation

F64L, S65T, Y66W, S72A, N146I, Y145A, H148D, M153T, V163A, A206K relative the wild type GFP sequence. The EGFP (Clontech Laboratories, Inc., Palo, Alto, CA) contains a valine immediately after the start codon that is not found in the wild type sequence. However, to avoid confusion with previously published work on GFP mutants, the wild type residue numbers are used.

name

VSVG

shRNA_sequence

size

1500

species