This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pEGFP-VSVG
catalog :
11912
citations: 33
Reference
Xu L, Tang C. Protocol for lentivirus-mediated delivery of genes to study neurogenesis and cognitive function in adult rodents. STAR Protoc. 2023;4:102761 pubmed publisher
Hirschenberger M, Lepelley A, Rupp U, Klute S, Hunszinger V, Koepke L, et al. ARF1 prevents aberrant type I interferon induction by regulating STING activation and recycling. Nat Commun. 2023;14:6770 pubmed publisher
Vierra N, Ribeiro Silva L, Kirmiz M, van der List D, Bhandari P, Mack O, et al. Neuronal ER-plasma membrane junctions couple excitation to Ca2+-activated PKA signaling. Nat Commun. 2023;14:5231 pubmed publisher
Bajaj R, Rodriguez B, Russell W, Warner A, Diao L, Wang J, et al. Impad1 and Syt11 work in an epistatic pathway that regulates EMT-mediated vesicular trafficking to drive lung cancer invasion and metastasis. Cell Rep. 2022;40:111429 pubmed publisher
Christensen J, Kendrick A, Truong J, Aguilar Maldonado A, Adani V, Dzieciatkowska M, et al. Cytoplasmic dynein-1 cargo diversity is mediated by the combinatorial assembly of FTS-Hook-FHIP complexes. elife. 2021;10: pubmed publisher
Hülsemann M, Sanchez C, Verkhusha P, Des Marais V, Mao S, Donnelly S, et al. TC10 regulates breast cancer invasion and metastasis by controlling membrane type-1 matrix metalloproteinase at invadopodia. Commun Biol. 2021;4:1091 pubmed publisher
Kerselidou D, Dohai B, Nelson D, Daakour S, De Cock N, Hassoun Z, et al. Alternative glycosylation controls endoplasmic reticulum dynamics and tubular extension in mammalian cells. Sci Adv. 2021;7: pubmed publisher
Perez Jimenez E, Viana R, Muñoz Ballester C, Vendrell Tornero C, Moll Diaz R, Garcia Gimeno M, et al. Endocytosis of the glutamate transporter 1 is regulated by laforin and malin: Implications in Lafora disease. Glia. 2020;: pubmed publisher
Van Bergen N, Ahmed S, Collins F, Cowley M, Vetro A, Dale R, et al. Mutations in the exocyst component EXOC2 cause severe defects in human brain development. J Exp Med. 2020;217: pubmed publisher
Westrate L, Hoyer M, Nash M, Voeltz G. Vesicular and uncoated Rab1-dependent cargo carriers facilitate ER to Golgi transport. J Cell Sci. 2020;133: pubmed publisher
Hao H, Niu J, Xue B, Su Q, Liu M, Yang J, et al. Golgi-associated microtubules are fast cargo tracks and required for persistent cell migration. EMBO Rep. 2020;21:e48385 pubmed publisher
Wei Z, Zhang M, Li C, Huang W, Fan Y, Guo J, et al. Specific TBC Domain-Containing Proteins Control the ER-Golgi-Plasma Membrane Trafficking of GPCRs. Cell Rep. 2019;28:554-566.e4 pubmed publisher
Subramanian A, Capalbo A, Iyengar N, Rizzo R, Di Campli A, Di Martino R, et al. Auto-regulation of Secretory Flux by Sensing and Responding to the Folded Cargo Protein Load in the Endoplasmic Reticulum. Cell. 2019;176:1461-1476.e23 pubmed publisher
Schips T, Vanhoutte D, Vo A, Correll R, Brody M, Khalil H, et al. Thrombospondin-3 augments injury-induced cardiomyopathy by intracellular integrin inhibition and sarcolemmal instability. Nat Commun. 2019;10:76 pubmed publisher
Shen S, Ji Y, Zhang C, Dong S, Yang S, Xiong Z, et al. Nuclear PTEN safeguards pre-mRNA splicing to link Golgi apparatus for its tumor suppressive role. Nat Commun. 2018;9:2392 pubmed publisher
Guo M, Chandris P, Giannini J, Trexler A, Fischer R, Chen J, et al. Single-shot super-resolution total internal reflection fluorescence microscopy. Nat Methods. 2018;15:425-428 pubmed publisher
Jalagadugula G, Goldfinger L, Mao G, Lambert M, Rao A. Defective RAB1B-related megakaryocytic ER-to-Golgi transport in RUNX1 haplodeficiency: impact on von Willebrand factor. Blood Adv. 2018;2:797-806 pubmed publisher
Dubois F, Alpha K, Turner C. Paxillin regulates cell polarization and anterograde vesicle trafficking during cell migration. Mol Biol Cell. 2017;28:3815-3831 pubmed publisher
Law K, Bronte Tinkew D, Di Pietro E, Snowden A, Jones R, Moser A, et al. The peroxisomal AAA ATPase complex prevents pexophagy and development of peroxisome biogenesis disorders. Autophagy. 2017;13:868-884 pubmed publisher
Wilhelmi I, Kanski R, Neumann A, Herdt O, Hoff F, Jacob R, et al. Sec16 alternative splicing dynamically controls COPII transport efficiency. Nat Commun. 2016;7:12347 pubmed publisher
Noda K, Kitami M, Kitami K, Kaku M, Komatsu Y. Canonical and noncanonical intraflagellar transport regulates craniofacial skeletal development. Proc Natl Acad Sci U S A. 2016;113:E2589-97 pubmed publisher
Nguyen M, Kim C, Kim J, Park B, Lee S, Park H, et al. Optogenetic oligomerization of Rab GTPases regulates intracellular membrane trafficking. Nat Chem Biol. 2016;12:431-6 pubmed publisher
Seggewiß N, Kruse H, Weilandt R, Domsgen E, Dotzauer A, Paulmann D. Cellular localization and effects of ectopically expressed hepatitis A virus proteins 2B, 2C, 3A and their intermediates 2BC, 3AB and 3ABC. Arch Virol. 2016;161:851-65 pubmed publisher
Wang C, Timmons C, Shao Q, Kinlock B, Turner T, Iwamoto A, et al. GB virus type C E2 protein inhibits human immunodeficiency virus type 1 Gag assembly by downregulating human ADP-ribosylation factor 1. Oncotarget. 2015;6:43293-309 pubmed publisher
Copeland S, Thurston S, Copeland J. Actin- and microtubule-dependent regulation of Golgi morphology by FHDC1. Mol Biol Cell. 2016;27:260-76 pubmed publisher
Hines W, Yaswen P, Bissell M. Modelling breast cancer requires identification and correction of a critical cell lineage-dependent transduction bias. Nat Commun. 2015;6:6927 pubmed publisher
Cho H, Yu J, Xie C, Rudrabhatla P, Chen X, Wu J, et al. Leucine-rich repeat kinase 2 regulates Sec16A at ER exit sites to allow ER-Golgi export. EMBO J. 2014;33:2314-31 pubmed publisher
Johns H, González López C, Sayers C, Hollinshead M, Elliott G. Rab6 dependent post-Golgi trafficking of HSV1 envelope proteins to sites of virus envelopment. Traffic. 2014;15:157-78 pubmed publisher
Dooley R, Angibaud E, Yusef Y, Thomas W, Harvey B. Aldosterone-induced ENaC and basal Na+/K+-ATPase trafficking via protein kinase D1-phosphatidylinositol 4-kinaseIII? trans Golgi signalling in M1 cortical collecting duct cells. Mol Cell Endocrinol. 2013;372:86-95 pubmed publisher
Olsen R, Echtenkamp F, Cheranova D, Deng W, Finlay B, Hardwidge P. The enterohemorrhagic Escherichia coli effector protein NleF binds mammalian Tmp21. Vet Microbiol. 2013;164:164-70 pubmed publisher
Mochizuki Y, Ohashi R, Kawamura T, Iwanari H, Kodama T, Naito M, et al. Phosphatidylinositol 3-phosphatase myotubularin-related protein 6 (MTMR6) is regulated by small GTPase Rab1B in the early secretory and autophagic pathways. J Biol Chem. 2013;288:1009-21 pubmed publisher
Conklin M, Ada Nguema A, Parsons M, Riching K, Keely P. R-Ras regulates beta1-integrin trafficking via effects on membrane ruffling and endocytosis. BMC Cell Biol. 2010;11:14 pubmed publisher
Presley J, Cole N, Schroer T, Hirschberg K, Zaal K, Lippincott Schwartz J. ER-to-Golgi transport visualized in living cells. Nature. 1997;389:81-5 pubmed
product information
Catalog Number :
11912
Product Name :
pEGFP-VSVG
article :
doi10.1038/38001
id689
pubmed_id9288971
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-N1
backbone_mutation
backbone_originClontech
backbone_size4733
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
The EGFP (Clontech Laboratories, Inc., Palo, Alto, CA) contains a valine immediately after the start codon that is not found in the wild type sequence. However, to avoid confusion with previously published work on GFP mutants, the wild type residue numbers are used. The Kozak sequence at the beginning of the GFP encoding region of the pEGFP-N1 plasmid from Clontech has been disrupted. Reference for the A206K mutation is Zacharias, D. A., Violin, J. D., Newton, A. C., and Tsien, R. Y. (2002) Science 296, 913-6.
origin :
37
pi :
alt_names
Vesicular stomatitus virus G protein
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5BglII
promoter
sequencing_primer_3
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutationF64L, S65T, A206K relative the wild type GFP sequence. The EGFP (Clontech Laboratories, Inc., Palo, Alto, CA) contains a valine immediately after the start codon that is not found in the wild type sequence. However, to avoid confusion with previously published work on GFP mutants, the wild type residue numbers are used.
nameVSVG
shRNA_sequence
size1500
species
tags
resistance markers :
294
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA