This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET-21a(+)-IPP1-His
catalog :
118978
citations: 1
Reference
Lavickova B, Maerkl S. A Simple, Robust, and Low-Cost Method To Produce the PURE Cell-Free System. ACS Synth Biol. 2019;8:455-462 pubmed publisher
product information
Catalog Number :
118978
Product Name :
pET-21a(+)-IPP1-His
article :
doi10.1021/acssynbio.8b00427
id28196835
pubmed_id30632751
bacterial resistance :
Ampicillin
cloning :
backbonepET21a(+)
backbone_mutationPart of the original backbone, containing the T7 promoter, the RBS and the T7 terminator, was replaced by the insert.
backbone_originEMD Biosciences
backbone_size5443
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Please visit https://www.biorxiv.org/content/early/2018/09/18/420570 for bioRxiv preprint.
growth strain :
Bacterial expression plasmid for Inorganic pyrophosphatase
origin :
37
pi :
alt_names
Inorganic pyrophosphatase
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterT7 promoter
sequencing_primer_3GCGTCCGGCGTAGAGGATC
sequencing_primer_5GCGTCCCATTCGCCAATCCG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYBR011C, PPA1
geneIPP1
id852296
genbank_ids
NM_001178359.1
mutation
nameinorganic diphosphatase IPP1
shRNA_sequence
size1376
species
4932
Saccharomyces cerevisiae
tags
locationC terminal on insert
tag6x-His
plasmid copy :
The gene was originally cloned to vector pET29b by Yoshihiro Shimizu (RIKEN). (Shimizu, Yoshihiro, et al. Cell-free translation reconstituted with purified components. Nature biotechnology 19.8 (2001): 751.)
resistance markers :
4217
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA