This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET-21a(+)-AK1-His
catalog :
118977
citations: 2
Reference
Libicher K, Hornberger R, Heymann M, Mutschler H. In vitro self-replication and multicistronic expression of large synthetic genomes. Nat Commun. 2020;11:904 pubmed publisher
Lavickova B, Maerkl S. A Simple, Robust, and Low-Cost Method To Produce the PURE Cell-Free System. ACS Synth Biol. 2019;8:455-462 pubmed publisher
product information
Catalog Number :
118977
Product Name :
pET-21a(+)-AK1-His
article :
doi10.1021/acssynbio.8b00427
id28196835
pubmed_id30632751
bacterial resistance :
Ampicillin
cloning :
backbonepET21a(+)
backbone_mutationPart of the original backbone, containing the T7 promoter, the RBS and the T7 terminator, was replaced by the insert.
backbone_originEMD Biosciences
backbone_size5443
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Please visit https://www.biorxiv.org/content/early/2018/09/18/420570 for bioRxiv preprint.
growth strain :
Bacterial expression plasmid for Adenylate kinase isoenzyme 1
origin :
37
pi :
alt_names
Myokinase
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterT7 promoter
sequencing_primer_3GCGTCCGGCGTAGAGGATC
sequencing_primer_5GCGTCCCATTCGCCAATCCG
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
geneAK1
id396002
genbank_ids
NM_205109.2
mutation
nameadenylate kinase isoenzyme 1 AK1
shRNA_sequence
size1097
species
9031
Gallus gallus
tags
locationC terminal on insert
tag6x-His
plasmid copy :
Gene was originally clone to vector pET29b by Yoshihiro Shimizu, RIKEN Center for Biosystems Dynamics Research
resistance markers :
4217
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA