This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBOB-Lck-GFP
catalog :
118738
citations: 1
Reference
Strating E, Verhagen M, Wensink E, D xfc nnebach E, Wijler L, Aranguren I, et al. Co-cultures of colon cancer cells and cancer-associated fibroblasts recapitulate the aggressive features of mesenchymal-like colon cancer. Front Immunol. 2023;14:1053920 pubmed publisher
product information
Catalog Number :
118738
Product Name :
pBOB-Lck-GFP
article :
doi
id28196799
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepCSC-SP-PW-GFP
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Lentiviral
growth notes :
Cloned membrane targeting Lck sequence from popular Addgene plasmid #61099. H8N mutation does not affect function.
growth strain :
To express membrane targeted GFP
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3Age I
cloning_site_5Xba I
promoterCMV
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesIMD22, LSK, YT16, p56lck, pp58lck
geneLCK
id3932
genbank_ids
mutationH8N (see comments)
nameLck domain
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationC terminal on insert
tagGFP
resistance markers :
91
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA