pLenti CMV TRE3G Neo GFP-Progerin C661S | Addgene 118711 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pLenti CMV TRE3G Neo GFP-Progerin C661S

catalog :

118711

citations: 1

Reference
Kubben N, Brimacombe K, Donegan M, Li Z, Misteli T. A high-content imaging-based screening pipeline for the systematic identification of anti-progeroid compounds. Methods. 2016;96:46-58 pubmed publisher

product information

Catalog Number :

118711

Product Name :

pLenti CMV TRE3G Neo GFP-Progerin C661S

article :

doi	10.1016/j.ymeth.2015.08.024
id	17108
pubmed_id	26341717

bacterial resistance :

Ampicillin

cloning :

backbone

pLenti CMV TRE3G Neomycin

backbone_mutation

backbone_origin

Eric Campeau

backbone_size

8013

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

Other

TET-ON inducible

growth notes :

To generate mammalian inducible cell lines co-infect with lentivirus made from: pLenti CMV rtTA3 Hygro (w785-1)( Addgene Plasmid #26730)

growth strain :

Lentiviral TET-ON inducible GFP-Progerin C661S (Farnesylation mutant)

growth temp :

Construct may be grown in DH5A cells, and or 37 degrees, though STBL3 and 30 degrees are recommended to reduce chance of recombination events.

origin :

pi :

alt_names

Progerin C661S

cloning

clone_method	Gateway Cloning
cloning_site_3
cloning_site_5
promoter	CMV TRE3G (TET-ON)
sequencing_primer_3	CATAGCGTAAAAGGAGCAACA
sequencing_primer_5	tgaccagtttactccctatcagtg
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, FPLD2, HGPS, IDC, LDP1, LFP, LGMD1B, LMN1, LMNC, LMNL1, MADA, PRO1
gene	LMNA
id	4000

genbank_ids

ENSG00000160789

mutation

C661S farnesylation mutant

name

GFP-Progerin C661S

shRNA_sequence

size

2632

species

9606

Homo sapiens