This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLenti CMV TRE3G Neo GFP-Progerin
catalog :
118710
citations: 2
Reference
Coll Bonfill N, Mahajan U, Shashkova E, Lin C, Mecham R, Gonzalo S. Progerin induces a phenotypic switch in vascular smooth muscle cells and triggers replication stress and an aging-associated secretory signature. GeroScience. 2022;: pubmed publisher
Kubben N, Brimacombe K, Donegan M, Li Z, Misteli T. A high-content imaging-based screening pipeline for the systematic identification of anti-progeroid compounds. Methods. 2016;96:46-58 pubmed publisher
product information
Catalog Number :
118710
Product Name :
pLenti CMV TRE3G Neo GFP-Progerin
article :
doi10.1016/j.ymeth.2015.08.024
id17108
pubmed_id26341717
bacterial resistance :
Ampicillin
cloning :
backbonepLenti CMV TRE3G Neomycin
backbone_mutation
backbone_originEric Campeau
backbone_size8013
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
Other
TET-ON inducible
growth notes :
To generate mammalian inducible cell lines co-infect with lentivirus made from: pLenti CMV rtTA3 Hygro (w785-1)( Addgene Plasmid #26730)
growth strain :
Lentiviral TET-ON inducible GFP-Progerin
growth temp :
Construct may be grown in DH5A cells, and or 37 degrees, though STBL3 and 30 degrees are recommended to reduce chance of recombination events.
origin :
30
pi :
alt_names
Progerin
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterCMV TRE3G (TET-ON)
sequencing_primer_3CATAGCGTAAAAGGAGCAACA
sequencing_primer_5tgaccagtttactccctatcagtg
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, FPLD2, HGPS, IDC, LDP1, LFP, LGMD1B, LMN1, LMNC, LMNL1, MADA, PRO1
geneLMNA
id4000
genbank_ids
ENSG00000160789
mutation
nameGFP-Progerin
shRNA_sequence
size2632
species
9606
Homo sapiens
tags
locationN terminal on insert
tagGFP
resistance markers :
343
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA