This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLenti CMV TRE3G Neo GFP-Lamin A wildtype
catalog :
118709
citations: 3
Reference
Coll Bonfill N, Mahajan U, Shashkova E, Lin C, Mecham R, Gonzalo S. Progerin induces a phenotypic switch in vascular smooth muscle cells and triggers replication stress and an aging-associated secretory signature. GeroScience. 2022;: pubmed publisher
Yan P, Li Z, Xiong J, Geng Z, Wei W, Zhang Y, et al. LARP7 ameliorates cellular senescence and aging by allosterically enhancing SIRT1 deacetylase activity. Cell Rep. 2021;37:110038 pubmed publisher
Kubben N, Brimacombe K, Donegan M, Li Z, Misteli T. A high-content imaging-based screening pipeline for the systematic identification of anti-progeroid compounds. Methods. 2016;96:46-58 pubmed publisher
product information
Catalog Number :
118709
Product Name :
pLenti CMV TRE3G Neo GFP-Lamin A wildtype
article :
doi10.1016/j.ymeth.2015.08.024
id17108
pubmed_id26341717
bacterial resistance :
Ampicillin
cloning :
backbonepLenti CMV TRE3G Neomycin
backbone_mutation
backbone_originEric Campeau
backbone_size8013
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
Other
TET-ON inducible
growth notes :
To generate mammalian inducible cell lines co-infect with lentivirus made from: pLenti CMV rtTA3 Hygro (w785-1)( Addgene Plasmid #26730)
growth strain :
Lentiviral TET-ON inducible GFP-Lamin A wildtype
growth temp :
Construct may be grown in DH5A cells, and or 37 degrees, though STBL3 and 30 degrees are recommended to reduce chance of recombination events.
origin :
30
pi :
alt_names
LMNA WT
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterCMV TRE3G (TET-ON)
sequencing_primer_3CATAGCGTAAAAGGAGCAACA
sequencing_primer_5tgaccagtttactccctatcagtg
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, FPLD2, HGPS, IDC, LDP1, LFP, LGMD1B, LMN1, LMNC, LMNL1, MADA, PRO1
geneLMNA
id4000
genbank_ids
ENSG00000160789
mutation
nameGFP-Lamin A human wildtype
shRNA_sequence
size2782
species
9606
Homo sapiens
tags
locationN terminal on insert
tagGFP
resistance markers :
343
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA