This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pMCh2007_pLenti_Syn_mChe-cdc42E7
catalog :
118620
citations: 1
Reference
Ciolli Mattioli C, Rom A, Franke V, Imami K, Arrey G, Terne M, et al. Alternative 3' UTRs direct localization of functionally diverse protein isoforms in neuronal compartments. Nucleic Acids Res. 2019;47:2560-2573 pubmed publisher
product information
Catalog Number :
118620
Product Name :
pMCh2007_pLenti_Syn_mChe-cdc42E7
article :
doi10.1093/nar/gky1270
id28196776
pubmed_id30590745
bacterial resistance :
Ampicillin
cloning :
backbonepLenti.Syn(0.5).H2B-eGFP.W
backbone_mutation
backbone_origin
backbone_size7846
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth strain :
expression of mChe-tagged cdc42E7 under pSyn promoter for primary neurons
origin :
37
pi :
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promotersynapsin I (rat)
sequencing_primer_3CAGGGAAGTAGCCTTGTGTGTG
sequencing_primer_5CGCACCACAAGAGGTGCAAGATAG
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
geneCdc42
id12540
genbank_ids
NM_009861
NM_009861
mutationchanged cagtctg to ggcataa (667 - 673 nt in 3'UTR) for shRNA resistance
namecdc42E7
shRNA_sequence
size1983
species
10090
Mus musculus
tags
locationN terminal on insert
tagmCherry
resistance markers :
3014
tags :
Low Copy
terms :
Other
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA