This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
CDK1as_T2A_puromycin
catalog :
118595
citations: 1
Reference
Saldivar J, Hamperl S, Bocek M, Chung M, Bass T, Cisneros Soberanis F, et al. An intrinsic S/G2 checkpoint enforced by ATR. Science. 2018;361:806-810 pubmed publisher
product information
Catalog Number :
118595
Product Name :
CDK1as_T2A_puromycin
article :
doi10.1126/science.aap9346
id28196772
pubmed_id30139873
bacterial resistance :
Ampicillin
cloning :
backbonepT2/SVNeo, a gift from Perry Hackett (Addgene 26553 Cui et al., 2002)
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Please also cite the following publication: Hochegger H, Dejsuphong D, Sonoda E, Saberi A, Rajendra E, Kirk J, Hunt T, Takeda S. An essential role for Cdk1 in S phase control is revealed via chemical genetics in vertebrate cells. J Cell Biol. 2007 Jul 16;178(2):257-68. PubMed PMID: 17635936; PubMed Central PMCID: PMC2064445.
growth strain :
One of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and puromycin resistance cassette flanked by Sleeping Beauty transposon.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3ApaI
cloning_site_5NruI
promoterCMV
sequencing_primer_3
sequencing_primer_5T7
site_3_destroyed1
site_5_destroyed1
entrez_gene
genbank_ids
mutation
nameCDK1
shRNA_sequence
size
species
8355
Xenopus laevis
tags
resistance markers :
4203
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA