This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
GFP-Braf R188L
catalog :
118330
citations: 1
Reference
Li Y, Takahashi M, Stork P. Ras-mutant cancer cells display B-Raf binding to Ras that activates extracellular signal-regulated kinase and is inhibited by protein kinase A phosphorylation. J Biol Chem. 2013;288:27646-57 pubmed publisher
product information
Catalog Number :
118330
Product Name :
GFP-Braf R188L
article :
doi10.1074/jbc.M113.463067
id28196745
pubmed_id23893412
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutation
backbone_origininvitrogen
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
site directed mutagenesis. BRaf constructs were subcloned into pcDNA3 containing GFP epitope with NotI and XbaI.
growth strain :
Expression of human Braf
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5NotI
promoterCMV
sequencing_primer_3
sequencing_primer_5EGFP-C
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesB-RAF1, B-raf, BRAF-1, BRAF1, NS7, RAFB1
geneBRAF
id673
genbank_ids
mutationR188L
nameBraf R188L
shRNA_sequence
size2301
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagGFP
resistance markers :
4189
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA