This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
GFP-RanV19 E1RA2
catalog :
118317
citations: 1
Reference
Liu C, Takahashi M, Li Y, Dillon T, Kaech S, Stork P. The interaction of Epac1 and Ran promotes Rap1 activation at the nuclear envelope. Mol Cell Biol. 2010;30:3956-69 pubmed publisher
product information
Catalog Number :
118317
Product Name :
GFP-RanV19 E1RA2
article :
doi10.1128/MCB.00242-10
id28196742
pubmed_id20547757
bacterial resistance :
Kanamycin
cloning :
backbonepeGFP-C1
backbone_mutation
backbone_originclontech
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
RanV19 was subcloned into GFP-Epac1RA2 with HindIII and EcoRI RA2 of Epac2 was inserted between aa 423 and 586 of Epac1.
growth strain :
Expression of human Ran
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5HindIII
promoter
sequencing_primer_3
sequencing_primer_5EGFP-C
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesARA24, Gsp1, TC4
geneRAN
id5901
genbank_ids
mutationin-frame insertion of RanV19 into GFPEpac1RA2
nameRanV19
shRNA_sequence
size3350
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagGFP
alt_names
Rapgef4
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5EcoRI
promoter
sequencing_primer_3
sequencing_primer_5n/a
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCAMP-GEFI, EPAC, EPAC1, HSU79275, bcm910
geneRAPGEF3
id10411
aliasesCAMP-GEFII, CGEF2, EPAC, EPAC 2, EPAC2, Nbla00496
geneRAPGEF4
id11069
genbank_ids
mutationsee comments
nameEPac1RA2
shRNA_sequence
size
species
9606
Homo sapiens
tags
resistance markers :
4189
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA