This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET15b-Casp8 delta DED
catalog :
11827
citations: 4
Reference
den Hamer A, Dierickx P, Arts R, de Vries J, Brunsveld L, Merkx M. Bright Bioluminescent BRET Sensor Proteins for Measuring Intracellular Caspase Activity. ACS Sens. 2017;2:729-734 pubmed publisher
Turowec J, Zukowski S, Knight J, Smalley D, Graves L, Johnson G, et al. An unbiased proteomic screen reveals caspase cleavage is positively and negatively regulated by substrate phosphorylation. Mol Cell Proteomics. 2014;13:1184-97 pubmed publisher
Halawani D, Tessier S, Anzellotti D, Bennett D, Latterich M, LeBlanc A. Identification of Caspase-6-mediated processing of the valosin containing protein (p97) in Alzheimer's disease: a novel link to dysfunction in ubiquitin proteasome system-mediated protein degradation. J Neurosci. 2010;30:6132-42 pubmed publisher
Zhou Q, Snipas S, Orth K, Muzio M, Dixit V, Salvesen G. Target protease specificity of the viral serpin CrmA. Analysis of five caspases. J Biol Chem. 1997;272:7797-800 pubmed
product information
Catalog Number :
11827
Product Name :
pET15b-Casp8 delta DED
article :
doi10.1074/jbc.272.12.7797
id668
pubmed_id9065443
bacterial resistance :
Ampicillin
cloning :
backbonepET-15 b
backbone_mutation
backbone_origin
backbone_size3666
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
origin :
37
pi :
alt_names
Caspase 8
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5NdeI
promoter
sequencing_primer_3
sequencing_primer_5T7 terminal primer
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesALPS2B, CAP4, Casp-8, FLICE, MACH, MCH5
geneCASP8
id841
genbank_ids
mutationMinus Death Defecter Domain (aa 1-216)
nameCaspase 8 minus Death Effecter Domain
shRNA_sequence
size926
species
9606
Homo sapiens
tags
locationN terminal on insert
tag6xHis
resistance markers :
435
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA