N295A (EcN), E166Q (EcN) MetNI LmH | Addgene 118269 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

N295A (EcN), E166Q (EcN) MetNI LmH

catalog :

118269

citations: 1

Reference
Nguyen P, Lai J, Lee A, Kaiser J, Rees D. Noncanonical role for the binding protein in substrate uptake by the MetNI methionine ATP Binding Cassette (ABC) transporter. Proc Natl Acad Sci U S A. 2018;115:E10596-E10604 pubmed publisher

product information

Catalog Number :

118269

Product Name :

N295A (EcN), E166Q (EcN) MetNI LmH

article :

doi	10.1073/pnas.1811003115
id	28196730
pubmed_id	30352853

bacterial resistance :

Ampicillin

cloning :

backbone

pET19b

backbone_mutation

Addd extra T7 Promoter, T7 Terminator, and 6x His Tag with the addition of MetN gene.

backbone_origin

Novagen

backbone_size

5453

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth strain :

N295A and E166Q Mutation of MetN in Methionine ABC Transporter with 6x His Tag. Used to solve crystal structure

origin :

pi :

ECK0198, JW0194, metD, yaeEgenemetIid944894genbank_ids

944894

mutationnameMetIshRNA_sequencesize654species

32644

Other

Escherichia coli

tags

location
tag	6x His Tag

alt_names

cloning

clone_method	Unknown
cloning_site_3
cloning_site_5
promoter	T7 Promoter
sequencing_primer_3	ctcgagtcagacataacccagtacctcta
sequencing_primer_5	tttaattcagttcgcaggcgaccgcatcg
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	b0199, ECK0199, JW0195, abc, metD
gene	metN
id	944896

genbank_ids

944896

mutation

Changed Glutamic Acid (Glu) 166 to Glutamine (Gln) and Changed Asparagine (Asn) 295 to Alanine (Ala)

name

MetN (E166Q ; N295A)

shRNA_sequence

size

1032

species

32644

Other

Escherichia coli