This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
N229A Lo-MetQ
catalog :
118268
citations: 1
Reference
Nguyen P, Lai J, Lee A, Kaiser J, Rees D. Noncanonical role for the binding protein in substrate uptake by the MetNI methionine ATP Binding Cassette (ABC) transporter. Proc Natl Acad Sci U S A. 2018;115:E10596-E10604 pubmed publisher
product information
Catalog Number :
118268
Product Name :
N229A Lo-MetQ
article :
doi10.1073/pnas.1811003115
id28196730
pubmed_id30352853
bacterial resistance :
Ampicillin
cloning :
backbonepET19b
backbone_mutation
backbone_originNovagen
backbone_size5652
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
N229A Mutation in Methionine Binding Protein (MetQ) used to solve crystal structure
origin :
37
pi :
alt_names
Methionine Binding Protein
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterT7 Promoter
sequencing_primer_3T7 Reverse
sequencing_primer_5T7 Forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesb0197, ECK0197, metD, yaeC
genemetQ
id944893
genbank_ids
944893
mutationChanged Asparagine (Asn) 229 to Alanine (Ala)
nameMetQ (N229A)
shRNA_sequence
size747
species
32644
Other
E. coli
tags
locationN terminal on insert
tag10x His Tag
locationN terminal on insert
tagEnterokinase Cleavage Site
resistance markers :
2861
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA