This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
N229A (EcQ) MetNIQ in pBAD
catalog :
118257
citations: 1
Reference
Nguyen P, Lai J, Lee A, Kaiser J, Rees D. Noncanonical role for the binding protein in substrate uptake by the MetNI methionine ATP Binding Cassette (ABC) transporter. Proc Natl Acad Sci U S A. 2018;115:E10596-E10604 pubmed publisher
product information
Catalog Number :
118257
Product Name :
N229A (EcQ) MetNIQ in pBAD
article :
doi10.1073/pnas.1811003115
id28196730
pubmed_id30352853
bacterial resistance :
Ampicillin
cloning :
backbonepBAD
backbone_mutation
backbone_originThermo Scientific Invitrogen
backbone_size3963
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
N229A Mutation in Methionine Binding Protein (MetQ) and Wild Type Methionine ABC Transporter for Transport Activity Assay
origin :
37
pi :
ECK0198, JW0194, metD, yaeEgenemetIid944894genbank_ids
944894
mutationnameMetIshRNA_sequencesize654species
32644
Other
E. coli
tags
alt_names
Methionine Binding Protein
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterNone
sequencing_primer_3pBAD Reverse
sequencing_primer_5TTTAATTCAGTTCGCAGGCGACCGCATCG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesb0197, ECK0197, metD, yaeC
genemetQ
id944893
genbank_ids
944893
mutationChanged Asparagine (Asn) 229 to Alanine (Ala)
nameMetQ (N229A)
shRNA_sequence
size813
species
32644
Other
E. coli
tags
locationC terminal on insert
tag6x His Tag

Site>able>alt_namesr>cloningd>clone_method>tr>

d>GCCGACTTTAATGTGGTTTGGATCTTT
d>
Unknown
cloning_site_3
cloning_site_5
promoterNone
sequencing_primer_3
sequencing_primer_5TAG
CGCGCAGATGGATTACGCCGGTGGCGTTAAGTTCGGCtd>
site_3_destroyed
site_5_destroyedd>
<
td>entrez_gene
<
table>
aliasesb0198,
alt_names
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoteraraBAD Promoter
sequencing_primer_3TTAGCAATAATTTGCCCCGGACGCGTGACATA
sequencing_primer_5pBAD Forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesb0199, ECK0199, JW0195, abc, metD
genemetN
id944896
genbank_ids
944896
mutationChanged Glutamic Acid (Glu) 295 to Alanine (Ala)
nameMetN
shRNA_sequence
size1032
species
32644
Other
E. coli
tags
locationN terminal on insert
tag10x HIs Tag
locationN terminal on insert
tagEnterokinase Cleavage
resistance markers :
2861
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA