This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
WT MetNI in pBAD
catalog :
118254
citations: 1
Reference
Nguyen P, Lai J, Lee A, Kaiser J, Rees D. Noncanonical role for the binding protein in substrate uptake by the MetNI methionine ATP Binding Cassette (ABC) transporter. Proc Natl Acad Sci U S A. 2018;115:E10596-E10604 pubmed publisher
product information
Catalog Number :
118254
Product Name :
WT MetNI in pBAD
article :
doi10.1073/pnas.1811003115
id28196730
pubmed_id30352853
bacterial resistance :
Ampicillin
cloning :
backbonepBAD
backbone_mutation
backbone_originThermo Scientific Invitrogen
backbone_size3966
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Wild Type Methionine ABC Transporter MetNI in pBAD Vector for Transport Activity Assay
origin :
37
pi :
ECK0198, JW0194, metD, yaeEgenemetIid944894genbank_ids
944894
mutationnameMetIshRNA_sequencesize654species
32644
Other
Escherichia coli
tags
Reversesequencing_pri
mer_5tagcgcgcagatggattacgccggt
ggcgttaagttcggcsite_3
_destroyedsi
te_5_destroyede>entrez_gene>
aliases<
/td>		b0198,
alt_names
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterArabinose Promoter
sequencing_primer_3ttagcaataatttgccccggacgcgtgacata
sequencing_primer_5pBAD Forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesb0199, ECK0199, JW0195, abc, metD
genemetN
id944896
genbank_ids
944896
mutation
nameMetN
shRNA_sequence
size1032
species
32644
Other
Escherichia coli
tags
locationN terminal on insert
tag10x His Tag
locationN terminal on insert
tagEnterokinase Cleavage Site
alt_names
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterNone
sequencing_primer_3pBAD
resistance markers :
2861
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA