This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET23b-Casp3-His
catalog :
11821
citations: 14
Reference
Hanna R, Rozenberg A, Saied L, Ben Yosef D, Lavy T, Kleifeld O. In-Depth Characterization of Apoptosis N-Terminome Reveals a Link Between Caspase-3 Cleavage and Posttranslational N-Terminal Acetylation. Mol Cell Proteomics. 2023;22:100584 pubmed publisher
Araya L, Soni I, Hardy J, Julien O. Deorphanizing Caspase-3 and Caspase-9 Substrates In and Out of Apoptosis with Deep Substrate Profiling. ACS Chem Biol. 2021;16:2280-2296 pubmed publisher
Soni I, Hardy J. Caspase-9 Activation of Procaspase-3 but Not Procaspase-6 Is Based on the Local Context of Cleavage Site Motifs and on Sequence. Biochemistry. 2021;60:2824-2835 pubmed publisher
Ruan Z, Orozco I, Du J, Lu W. Structures of human pannexin 1 reveal ion pathways and mechanism of gating. Nature. 2020;: pubmed publisher
Halawani D, Gogonea V, DiDonato J, Pipich V, Yao P, China A, et al. Structural control of caspase-generated glutamyl-tRNA synthetase by appended noncatalytic WHEP domains. J Biol Chem. 2018;293:8843-8860 pubmed publisher
Huber K, Serrano B, Hardy J. Caspase-9 CARD : core domain interactions require a properly formed active site. Biochem J. 2018;475:1177-1196 pubmed publisher
Smith C, Soti S, Jones T, Nakagawa A, Xue D, Yin H. Non-steroidal Anti-inflammatory Drugs Are Caspase Inhibitors. Cell Chem Biol. 2017;24:281-292 pubmed publisher
Hwang D, Kim S, Yang E, Song H, Chung H. A facile method to prepare large quantities of active caspase-3 overexpressed by auto-induction in the C41(DE3) strain. Protein Expr Purif. 2016;126:104-108 pubmed publisher
Qian L, Bradford A, Cooke P, Lyons B. Grb7 and Hax1 may colocalize partially to mitochondria in EGF-treated SKBR3 cells and their interaction can affect Caspase3 cleavage of Hax1. J Mol Recognit. 2016;29:318-33 pubmed publisher
Waldron Roby E, Hoerauf J, Arbez N, Zhu S, KULCSAR K, Ross C. Sox11 Reduces Caspase-6 Cleavage and Activity. PLoS ONE. 2015;10:e0141439 pubmed publisher
Heinemann D, Kalies S, Schomaker M, Ertmer W, Murua Escobar H, Meyer H, et al. Delivery of proteins to mammalian cells via gold nanoparticle mediated laser transfection. Nanotechnology. 2014;25:245101 pubmed publisher
Turowec J, Zukowski S, Knight J, Smalley D, Graves L, Johnson G, et al. An unbiased proteomic screen reveals caspase cleavage is positively and negatively regulated by substrate phosphorylation. Mol Cell Proteomics. 2014;13:1184-97 pubmed publisher
Halawani D, Tessier S, Anzellotti D, Bennett D, Latterich M, LeBlanc A. Identification of Caspase-6-mediated processing of the valosin containing protein (p97) in Alzheimer's disease: a novel link to dysfunction in ubiquitin proteasome system-mediated protein degradation. J Neurosci. 2010;30:6132-42 pubmed publisher
Zhou Q, Snipas S, Orth K, Muzio M, Dixit V, Salvesen G. Target protease specificity of the viral serpin CrmA. Analysis of five caspases. J Biol Chem. 1997;272:7797-800 pubmed
product information
Catalog Number :
11821
Product Name :
pET23b-Casp3-His
article :
doi10.1074/jbc.272.12.7797
id668
pubmed_id9065443
bacterial resistance :
Ampicillin
cloning :
backbonepET-23 b
backbone_mutation
backbone_origin
backbone_size3666
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5NdeI
promoter
sequencing_primer_3T7 terminal primer
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCPP32, CPP32B, SCA-1
geneCASP3
id836
genbank_ids
mutation
nameCaspase 3
shRNA_sequence
size999
species
9606
Homo sapiens
tags
locationC terminal on insert
tag6xHis
resistance markers :
435
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA