pCMV5B-Flag-Smurf1 C699A | Addgene 11753 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCMV5B-Flag-Smurf1 C699A

catalog :

11753

citations: 3

Reference
Werner C, Viswanathan R, Martin J, Gobira P, Mitra S, Thomas S, et al. E3 Ubiquitin-Protein Ligase SMURF1 in the Nucleus Accumbens Mediates Cocaine Seeking. Biol Psychiatry. 2018;84:881-892 pubmed publisher
Takebayashi Suzuki K, Konishi H, Miyamoto T, Nagata T, Uchida M, Suzuki A. Coordinated regulation of the dorsal-ventral and anterior-posterior patterning of Xenopus embryos by the BTB/POZ zinc finger protein Zbtb14. Dev Growth Differ. 2018;60:158-173 pubmed publisher
Zhu H, Kavsak P, Abdollah S, Wrana J, Thomsen G. A SMAD ubiquitin ligase targets the BMP pathway and affects embryonic pattern formation. Nature. 1999;400:687-93 pubmed

product information

Catalog Number :

11753

Product Name :

pCMV5B-Flag-Smurf1 C699A

article :

doi	10.1038/23293
id	654
pubmed_id	10458166

bacterial resistance :

Ampicillin

cloning :

backbone

pCMV5B

backbone_mutation

backbone_origin

backbone_size

4700

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

A human Smurf1 cDNA encoding all but the first eight amino acids was identified in the expressed-sequence-tag database (AA292123), and full-length hSmurf1 was reconstituted using a fragment of XSmurf1 cDNA encoding the first eight amino acids.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	XbaI
cloning_site_5	ClaI
promoter
sequencing_primer_3
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases
gene	SMURF1
id	57154

genbank_ids

NM_020429

mutation

C699A: catalytically inactive

name

SMURF1

shRNA_sequence

size

2300

species

9606

Homo sapiens