This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCMV5B-Flag-Smurf1 wt
catalog :
11752
citations: 7
Reference
Liu H, Du H, Khabibullin D, Zarei M, Wei K, Freeman G, et al. mTORC1 upregulates B7-H3/CD276 to inhibit antitumor T cells and drive tumor immune evasion. Nat Commun. 2023;14:1214 pubmed publisher
Kim S, Ham S, Yang K, Kim K. Protein kinase CK2 activation is required for transforming growth factor β-induced epithelial-mesenchymal transition. Mol Oncol. 2018;12:1811-1826 pubmed publisher
Werner C, Viswanathan R, Martin J, Gobira P, Mitra S, Thomas S, et al. E3 Ubiquitin-Protein Ligase SMURF1 in the Nucleus Accumbens Mediates Cocaine Seeking. Biol Psychiatry. 2018;84:881-892 pubmed publisher
Malonis R, Fu W, Jelcic M, Thompson M, Canter B, Tsikitis M, et al. RNF11 sequestration of the E3 ligase SMURF2 on membranes antagonizes SMAD7 down-regulation of transforming growth factor β signaling. J Biol Chem. 2017;292:7435-7451 pubmed publisher
Bouin A, Kyumurkov A, Régent Kloeckner M, Ribba A, Faurobert E, Fournier H, et al. ICAP-1 monoubiquitylation coordinates matrix density and rigidity sensing for cell migration through ROCK2-MRCK? balance. J Cell Sci. 2017;130:626-636 pubmed publisher
Chesarino N, McMichael T, Yount J. E3 Ubiquitin Ligase NEDD4 Promotes Influenza Virus Infection by Decreasing Levels of the Antiviral Protein IFITM3. PLoS Pathog. 2015;11:e1005095 pubmed publisher
Zhu H, Kavsak P, Abdollah S, Wrana J, Thomsen G. A SMAD ubiquitin ligase targets the BMP pathway and affects embryonic pattern formation. Nature. 1999;400:687-93 pubmed
product information
Catalog Number :
11752
Product Name :
pCMV5B-Flag-Smurf1 wt
article :
doi10.1038/23293
id654
pubmed_id10458166
bacterial resistance :
Ampicillin
cloning :
backbonepCMV5B
backbone_mutation
backbone_origin
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
A human Smurf1 cDNA encoding all but the first eight amino acids was identified in the expressed-sequence-tag database (AA292123), and full-length hSmurf1 was reconstituted using a fragment of XSmurf1 cDNA encoding the first eight amino acids.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5ClaI
promoter
sequencing_primer_3
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
geneSMURF1
id57154
genbank_ids
NM_020429
mutation
nameSMURF1
shRNA_sequence
size2300
species
9606
Homo sapiens
tags
locationN terminal on insert
tagFlag
resistance markers :
589
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA